On my desk to read today--another paper related to CRISPRs.
Montague TG, Cruz JM, Gagnon JA, Church GM, Valen E. CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing. Nucleic Acids Res. 2014 Jul;42(Web Server issue):W401-7. PMID: 24861617.
This report describes the ChopChop suite of tools, which includes support for Drosophila sgRNA design.
The DRSC offers our CRISPR2 sgRNA design tool, which provides a genome-browse view and includes efficiency scores (or you can run any designs through a 'calculator').
Additional tools are listed at AddGene.
The DRSC offers support for sgRNA and donor construct building through the Genome Engineering Production Group.
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