Thursday, October 16, 2014

G3 report--two-step CRISPR approach

Another fly gene editing paper early online at G3--

Xu Zhang, Wouter H. Koolhaas and Frank Schnorrer (2014) A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3. Early online.

Saturday, October 11, 2014

G3 report--plasmids for CRISPR-Cas9 in flies

Gokcezade J, Sienski G, Duchek P. Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila. G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. PMID: 25236734.

In the Acknowledgements the authors note that "The vectors described in this study (pDCC5 and pDCC6) will be available from Addgene and the Vienna Drosophila Resource Center (VDRC).

Thursday, October 9, 2014

Localization of CPTI collection of YFP-trap proteins

Lye CM, Naylor HW, Sanson B. Subcellular localisations of the CPTI collection of YFP-tagged proteins in Drosophila embryos. Development. 2014 Oct;141(20):4006-17. PMID: 25294944.

From the abstract:  "... The Cambridge Protein Trap Consortium generated, via piggyBac transposition, over 600 novel YFP-trap proteins tagging just under 400 Drosophila loci. Here, we characterise the subcellular localisations and expression patterns of these insertions, called the CPTI lines, in Drosophila embryos. ..."

MiMIC-based approach to gene knock-in

Early online at G3 --

Sven Vilain, Roeland Vanhauwaert, Ine Maes, Nils Schoovaerts, Lujia Zhou, Sandra Soukup, Raquel da Cunha, Elsa Lauwers, Mark Fiers, and Patrik Verstreken Fast and Efficient Drosophila melanogaster Gene Knock-Ins Using MiMIC Transposons
G3 early online October 8, 2014,

Wednesday, October 1, 2014

Drosophila cell-based genome-wide screen related to Parkinson's disease

Ivatt RM, Sanchez-Martinez A, Godena VK, Brown S, Ziviani E, Whitworth AJ. Genome-wide RNAi screen identifies the Parkinson disease GWAS risk locus SREBF1 as a regulator of mitophagy. Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8494-9. PMID: 24912190; PubMed Central PMCID: PMC4060696.  

From the abstract: "... We undertook a genome-wide RNAi screen as an unbiased approach to identify genes regulating the PINK1/Parkin pathway. We identified several genes that have a conserved function in promoting mitochondrial translocation of Parkin and subsequent mitophagy, most notably sterol regulatory element binding transcription factor 1 (SREBF1), F-box and WD40 domain protein 7 (FBXW7), and other components of the lipogenesis pathway. ..."

Saturday, September 27, 2014

Drosophila Models of Human Disease: Large-scale study of genes on the fly X-chromosome...

Drosophila Models of Human Disease: Large-scale study of genes on the fly X-chromosome...: Yamamoto S, Jaiswal M, Charng WL, Gambin T, Karaca E, Mirzaa G, Wiszniewski W, Sandoval H, Haelterman NA, Xiong B, Zhang K, Bayat V, David G...

Study related to Abl kinase uses primary cultured cells

This is not an RNAi study but the approach taken might of interest to those thinking about cell-based studies. Liu L, Wu CF. Distinct effects of Abelson kinase mutations on myocytes and neurons in dissociated Drosophila embryonic cultures: mimicking of high temperature. PLoS One. 2014 Jan 21;9(1):e86438. PMID: 24466097; PMCID: PMC3897706. From the abstract: "... we observed myocyte fusion events and nerve-muscle contact formation between WT and Abl cells in mixed WT and Abl cultures derived from labeled embryos ..."