Monday, July 28, 2014

Genome-wide, high-content image-based screen preformed at DRSC--identification of regulators of organization of polycomb foci

Gonzalez I, Mateos-Langerak J, Thomas A, Cheutin T, Cavalli G. Identification of regulators of the three-dimensional polycomb organization by a microscopy-based genome-wide RNAi screen. Mol Cell. 2014 May 8;54(3):485-99. PMID: 24703951.  

From the abstract: "... Here, we report the results of a high-resolution microscopy genome-wide RNAi screen that identifies 129 genes that regulate the nuclear organization of Pc foci. Candidate genes include PcG components and chromatin factors, as well as many protein-modifying enzymes, including components of the SUMOylation pathway. ... suggesting that the dynamic regulation of Pc SUMOylation regulates PcG-mediated silencing by modulating the kinetics of Pc binding to chromatin as well as its ability to form Polycomb foci."

Thursday, July 24, 2014

Cell-based assays related to Notch signaling

Xu A, Irvine KD. Notch-ligand binding assays in Drosophila cells. Methods Mol Biol. 2014;1187:277-84. PMID: 25053497.

 Li J, Housden BE, Bray SJ. Notch signaling assays in Drosophila cultured cell lines. Methods Mol Biol. 2014;1187:131-41. PMID: 25053486.

Monday, July 21, 2014

RNA biology--viruses & RNAi suppression

van Mierlo JT, Overheul GJ, Obadia B, van Cleef KW, Webster CL, Saleh MC, Obbard DJ, van Rij RP. Novel Drosophila Viruses Encode Host-Specific Suppressors of RNAi. PLoS Pathog. 2014 Jul 7;10(7):e1004256. PMID: 25032815.

Genetic manipulation of both host cells and pathogen--"iMAD" protocol

O'Connor TJ, Isberg RR. iMAD, a genetic screening strategy for dissecting complex interactions between a pathogen and its host. Nat Protoc. 2014 Aug;9(8):1916-30. PMID: 25033208.

From the abstract: "... Here we present a protocol for dissecting the interaction between a pathogen (Legionella pneumophila) and its host (cultured Drosophila melanogaster cells) using bacterial mutagenesis and host RNAi. ..."

Sunday, July 20, 2014

Genome-wide RNAi screen identifies neurons involved in female receptivity behavior

Bussell JJ, Yapici N, Zhang SX, Dickson BJ, Vosshall LB. Abdominal-B Neurons Control Drosophila Virgin Female Receptivity. Curr Biol. 2014 Jul 3. pii: S0960-9822(14)00689-7. PMID: 24998527.

From the abstract: "... Using a genome-wide neuronal RNAi screen, we identify a subpopulation of neurons responsible for pausing, a novel behavioral aspect of virgin female receptivity characterized in this study. ..."

in vivo RNAi screen related to Notch signaling in follicle cells

Xie G, Yu Z, Jia D, Jiao R, Deng WM. E(y)1/TAF9 mediates the transcriptional output of Notch signaling in Drosophila. J Cell Sci. 2014 Jul 11. PMID: 25015288.

From the abstract: "... We identified E(y)1/TAF9 in a large-scale in vivo RNAi screen for genes involved in a Notch-dependent mitotic-to-endocycle transition in Drosophila follicle cells. Knockdown of e(y)1/TAF9 displayed Notch-like phenotypes ..."

Fly RNAi screen of about 150 genes included in obesity study

Lee JH, Bassel-Duby R, Olson EN. Heart- and muscle-derived signaling system dependent on MED13 and Wingless controls obesity in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9491-6. PMID: 24979807.